Protein-coding gene in the species Homo sapiens
Mediator of RNA polymerase II transcription subunit 23 is an enzyme that in humans is encoded by the MED23 gene .[ 5] [ 6]
Function
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor . Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.[ 6]
Interactions
CRSP3 has been shown to interact with Estrogen receptor alpha ,[ 7] CEBPB [ 8] Cyclin-dependent kinase 8 .[ 7] [ 9]
References
^ a b c GRCh38: Ensembl release 89: ENSG00000112282 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000019984 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature . 397 (6718): 446–50. Bibcode :1999Natur.397..446R . doi :10.1038/17141 . hdl :11858/00-001M-0000-0019-A36A-8 PMID 9989412 . S2CID 4405569 . ^ a b "Entrez Gene: CRSP3 cofactor required for Sp1 transcriptional activation, subunit 3, 130kDa" .^ a b Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro" . Proceedings of the National Academy of Sciences of the United States of America . 99 (5): 2642–7. Bibcode :2002PNAS...99.2642K . doi :10.1073/pnas.261715899 PMC 122401 PMID 11867769 . ^ Mo X, Kowenz-Leutz E, Xu H, Leutz A (Jan 2004). "Ras induces mediator complex exchange on C/EBP beta" . Molecular Cell . 13 (2): 241–50. doi :10.1016/S1097-2765(03)00521-5 PMID 14759369 . ^ Wang G, Cantin GT, Stevens JL, Berk AJ (Jul 2001). "Characterization of mediator complexes from HeLa cell nuclear extract" . Molecular and Cellular Biology . 21 (14): 4604–13. doi :10.1128/MCB.21.14.4604-4613.2001 . PMC 87123 PMID 11416138 .
External links
Further reading
Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Näär AM, Erdjument-Bromage H, Tempst P, Freedman LP (Apr 1999). "Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex". Nature . 398 (6730): 824–8. Bibcode :1999Natur.398..824R . doi :10.1038/19783 . PMID 10235266 . S2CID 204992765 . Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R (Apr 1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature . 398 (6730): 828–32. Bibcode :1999Natur.398..828N . doi :10.1038/19789 . PMID 10235267 . S2CID 23646963 . Boyer TG, Martin ME, Lees E, Ricciardi RP, Berk AJ (May 1999). "Mammalian Srb/Mediator complex is targeted by adenovirus E1A protein". Nature . 399 (6733): 276–9. Bibcode :1999Natur.399..276B . doi :10.1038/20466 . PMID 10353252 . S2CID 4400507 . Nagase T, Ishikawa K, Kikuno R, Hirosawa M, Nomura N, Ohara O (Oct 1999). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro" . DNA Research . 6 (5): 337–45. doi :10.1093/dnares/6.5.337 PMID 10574462 . Ko L, Cardona GR, Chin WW (May 2000). "Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator" . Proceedings of the National Academy of Sciences of the United States of America . 97 (11): 6212–7. Bibcode :2000PNAS...97.6212K . doi :10.1073/pnas.97.11.6212 PMC 18584 PMID 10823961 . Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination" . Genome Research . 10 (11): 1788–95. doi :10.1101/gr.143000 . PMC 310948 PMID 11076863 . Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs" . Genome Research . 11 (3): 422–35. doi :10.1101/gr.GR1547R . PMC 311072 PMID 11230166 . Stevens JL, Cantin GT, Wang G, Shevchenko A, Shevchenko A, Berk AJ (Apr 2002). "Transcription control by E1A and MAP kinase pathway via Sur2 mediator subunit" . Science . 296 (5568): 755–8. Bibcode :2002Sci...296..755S . doi :10.1126/science.1068943 PMID 11934987 . S2CID 19392260 . Asada S, Choi Y, Yamada M, Wang SC, Hung MC, Qin J, Uesugi M (Oct 2002). "External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator" . Proceedings of the National Academy of Sciences of the United States of America . 99 (20): 12747–52. Bibcode :2002PNAS...9912747A . doi :10.1073/pnas.202162199 PMC 130531 PMID 12242338 . Nakayama M, Kikuno R, Ohara O (Nov 2002). "Protein-protein interactions between large proteins: two-hybrid screening using a functionally classified library composed of long cDNAs" . Genome Research . 12 (11): 1773–84. doi :10.1101/gr.406902 . PMC 187542 PMID 12421765 . Goldberg SF, Miele ME, Hatta N, Takata M, Paquette-Straub C, Freedman LP, Welch DR (Jan 2003). "Melanoma metastasis suppression by chromosome 6: evidence for a pathway regulated by CRSP3 and TXNIP". Cancer Research . 63 (2): 432–40. PMID 12543799 . Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (Dec 2003). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex" . The Journal of Biological Chemistry . 278 (50): 49671–4. doi :10.1074/jbc.C300444200 PMID 14576168 . Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (Feb 2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2" . The Journal of Biological Chemistry . 279 (7): 5846–51. doi :10.1074/jbc.M312523200 PMID 14638676 . Mo X, Kowenz-Leutz E, Xu H, Leutz A (Jan 2004). "Ras induces mediator complex exchange on C/EBP beta" . Molecular Cell . 13 (2): 241–50. doi :10.1016/S1097-2765(03)00521-5 PMID 14759369 . Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology" . Molecular Cell . 14 (5): 685–91. doi :10.1016/j.molcel.2004.05.006 PMID 15175163 . Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (Oct 2004). "From ORFeome to biology: a functional genomics pipeline" . Genome Research . 14 (10B): 2136–44. doi :10.1101/gr.2576704 . PMC 528930 PMID 15489336 .
