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Epitope binning
Epitope binning is a competitive immunoassay used to characterize and then sort a library of monoclonal antibodies against a target protein.[1] Antibodies against a similar target are tested against all other antibodies in the library in a pairwise fashion to see if antibodies block one another's binding to the epitope of an antigen.[2] After each antibody has a profile created against all of the other antibodies in the library, a competitive blocking profile is created for each antibody relative to the others in the library. Closely related binning profiles indicate that the antibodies have the same or a closely related epitope and are "binned" together.[2] Epitope binning is referenced in the literature under different names such as epitope mapping and epitope characterization.[2] Regardless of the naming, epitope binning is prevalent in the pharmaceutical industry.[2] Epitope Binning is used in the discovery and development of new therapeutics, vaccines, and diagnostics.[3] See alsoReferences
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